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| Registros recuperados: 10 | |
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| Menu, Bruno; Peruzzi, Stefano; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice. |
| The objective of this study was to develop a rapid and effective method of sexing juvenile sea bass with minimum labour and material. To this end, the gonad squash mount technique was applied along with macroscopic techniques for sexing a large number of experimental fish at the age of 215-275 days post fertilization (p.f.). At this age, 90% of the 3894 fish could be unambiguously sexed by macroscopic examination of their gonads, whereas the remaining proportion was identified using squash mount preparations (10%). The accuracy of the observations was measured up to classical histology procedures. Undifferentiated fish accounted for 0.1% of the total population only. The application of the squash mount technique holds out the prospect of considerable... |
| Tipo: Text |
Palavras-chave: Histology; Squash; Juvenile; Gonads; Seabass; Dicentrarchus labrax. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-378.pdf |
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| Peruzzi, Stefano; Wiborg-dahle, Stine; Haffray, Pierrick; Chatain, Beatrice; Primicerio, Raul. |
| A method of geometric morphometrics was used to characterize body shape variation in meiotic gynogenetic and triploid sea bass in comparison with diploid controls. A total of 13 coordinate landmark data were recorded on the profile of 34-month-old control (n = 81), gynogenetic (n = 117) and triploid (n = 131) siblings generated from two females (A and B). Three main axes of morphometric variation were consistent, and these accounted for 60.8 and 57.6% of the total variability in females A and B, respectively. There were significant shape deviations of triploid and gynogenetic fish relative to the controls, and differences were particularly evident in the progeny of one female. Overall, triploids displayed a more slender shape than diploids, whereas... |
| Tipo: Text |
Palavras-chave: Body shape variability; Morphometry; Geometry of Procrustes distance; Triploidy; Gynogenesis; European sea bass; Dicentrarchus labrax. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00025/13651/10757.pdf |
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| Peruzzi, Stefano; Chatain, Beatrice; Menu, Bruno. |
| The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant... |
| Tipo: Text |
Palavras-chave: Anguilla anguilla; Liza ramada; Sparus aurata; Dicentrarchus labrax; Teleosts; DNA content. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-411.pdf |
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| Oral, Munevver; Colleter, Julie; Bekaert, Michhttps://w3.ifremer.fr/archimer-admin/author.jsp#ael; Taggart, John B.; Palaiokostas, Christos; Mcandrew, Brendan J.; Vandeputte, Marc; Chatain, Beatrice; Kuhl, Heiner; Reinhardt, Richard; Peruzzi, Stefano; Penman, David J.. |
| Background Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. Results From a single meiotic gynogenetic family consisting of 79 progeny, 42... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Meiotic gynogenesis; Isogenic lines; DdRAD seq; Genetic map; Gene-Centromere map; Aquaculture. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00388/49901/50462.pdf |
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| Colleter, Julie; Penman, David J.; Lallement, Stephane; Fauvel, Christian; Hanebrekke, Tanja; Osvik, Renate D.; Eilertsen, Hans C.; D'Cotta, Helena; Chatain, Beatrice; Peruzzi, Stefano. |
| Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (gamma), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities.... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00239/34996/33531.pdf |
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| Peruzzi, Stefano; Westgaard, Jon-ivar; Chatain, Beatrice. |
| Although the actiology of swimbladder inflation anomalies in important aquaculture species such as European sea bass D. labrax is not fully determined, culture conditions are commonly suggested as main contributory factors. Little information is available on whether swimbladder inflation has a genetic basis for its expression too. In this work, 24 full-sibling sea bass families from a 4 dams x 6 sires factorial crossing were reared under communal conditions. The larvae developing normal and abnormal (uninflated or hyper-inflated) swimbladders were genotyped at four microsatellite loci, Labrax-3, Labrax-13, Labrax-17, Labrax29, and allocated to the individual breeders. Out of 273 offspring, 97% could be assigned to a single parental pair. The genotype and... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Genetics; Anomalies; Swimbladder. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2590.pdf |
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| Peruzzi, Stefano; Varsamos, Stamatis; Chatain, Beatrice; Fauvel, Christian; Menu, Bruno; Falguiere, Jean-claude; Severe, Armelle; Flik, Gert. |
| The purpose of this study was to determine whether diploid and triploid sea bass differed in terms of main haematological and physiological characteristics. Diploid and triploid fish were produced by sub-optimal pressure treatments and held in communal environments under standard rearing conditions. Total red blood cell count (RBCC), haemoglobin concentration (Hb), hematocrit (Hct), mean cell volume (MCV), mean cellular haemoglobin content (MCH), mean cell haemoglobin concentration (MCHC), plasma metabolites, osmotic pressure, gill Na+/K+-ATPase activity, electrolytes, cortisol, and 3,5,3'-triiodo-L-thyronine (T-3) were measured and compared. Triploidisation in sea bass led to an increase in erythrocyte size (32% in cytoplasm surface area, and 50% in... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Triploid; Diploid; Physiology; Haematology. |
| Ano: 2005 |
URL: https://archimer.ifremer.fr/doc/2005/publication-508.pdf |
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| Deslous-paoli, Jean-marc; Dosdat, Antoine; Peruzzi, Stefano. |
| The demand for sea products is going only to increase in the years to come. Capture fisheries will not be enough (80 million tons / year) to supply and fish farming (38 million tons of products o fthe sea in 1998) already stands out to itself as a dominating actor. One of the missions of Ifremer in the field of the fish farming is the harmonious development of the field by mastering possible impacts due to rearing. The Institute developed competence in immunology, physiology, genetics, pathology and food. This study report of impact on the useful bases of physic and biology to elaborate the impact studies in marine fish farming, within the framework of the french law on the Classified Installations for Environmental Protection (ICPE), confined in the... |
| Tipo: Text |
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| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00059/17022/14526.pdf |
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| Peruzzi, Stefano; Chatain, Beatrice. |
| The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0-1 degrees C for 15-20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm(-2)) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was... |
| Tipo: Text |
Palavras-chave: Parental variability; Pressure and cold shocks; Dicentrarchus labrax; Sea bass; Triploidy; Meiotic gynogenesis. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-507.pdf |
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| Monaco, André; Peruzzi, Stefano. |
| The papers presented in this volume represent only a small part of the results obtained within the scope of the first integrated programme concerning the Mediterranean Sea. The latter is considered as a downscaled model of the ocean system, and a regional sea for the targeted projects supported by the European Commission. Whatever the domain is, the variability of the system appeared as a major feature. Consequently, this theme has been selected, among the main initial objectives, as the central subject of this volume. Spatial and temporal variabilities are considered, at different scales and at different (water, particles, sediment, trophic) levels of the marine ecosystem. In the hereafter contributions, one will find the long-term (millenar, secular and... |
| Tipo: Text |
Palavras-chave: Climate; Pluridisciplinary; Variability; Mediterranean; Marine system. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-783.pdf |
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| Registros recuperados: 10 | |
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